Confocal Microscopy for Biologists

This manual is intended to teach its readers the basic concepts of microscopy, fluorescence, digital imaging, and the principles of confocal microscopy so that they may take full advantage of the excellent confocal microscopes now available. This manual is an excellent reference source for information related to confocal microscopy for both beginners and more advanced users. The main emphasis in this manual in on the laser scanning confocal microscopes used in biology. However, a variety of related imaging technologies are briefly introduced as a comparison.