Identification of Bacillus producing thermotolerant hydrolases

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The study focuses on the production of thermotolerant enzymes from various Bacillus species isolated from soil and water. Key enzymes such as protease, amylase, lipase, cellulase, and chitinase were produced under specific conditions at 50°C. Notably, B. polymyxa strains excelled in producing protease, amylase, and cellulase, while B. lentus was identified as an effective chitinase producer. Multiple Bacillus strains, including B. circulans and B. stearothermophilus, contributed to lipase production, highlighting the potential of these microorganisms for biotechnological applications.