Este trabajo investiga la interacción entre el ARN TAR del VIH-1 y la proteína viral Tat, buscando diseñar ligandos específicos como antagonistas antivirales. Se sintetizan ligandos de ARN y se analizan sus afinidades de unión y propiedades antivirales utilizando métodos como fluorescencia y RMN.
The research focuses on the interaction between HIV-1 TAR RNA and the viral protein Tat, crucial for viral genome transcription. By synthesizing RNA ligands using heteroaromatic building blocks, the study aims to develop specific antiviral Tat antagonists. Binding affinities were assessed through fluorescence assays and spectroscopy, revealing conformational changes in TAR RNA via NMR and molecular dynamics. Additionally, the antiviral effects of peptides and small molecules were evaluated using HeLa P4 cells, with a particular emphasis on diaminopyrazoles and indazoles.