Methods in non-aqueous enzymology

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Extending enzymatic catalysis into non-aqueous media has become a significant approach in biochemistry and biotechnology. Unlike conventional enzymology in aqueous buffers, understanding the parameters that influence enzyme behavior in non-aqueous environments has developed slowly. This lack of knowledge has made some researchers hesitant to adopt this method in their labs, hindering broader application. To promote the practice of non-aqueous enzymology, the availability of well-defined protocols for various enzyme applications is essential. This book provides such protocols, each accompanied by background material. The initial chapters cover general topics like controlling water activity and stabilization through immobilization. Subsequent chapters focus on specific transformations involving lipids, carbohydrates, peptide synthesis, and the preparation of chiral compounds. The emphasis on lipases is intentional, as this class of enzymes is frequently utilized in non-aqueous enzymology. By offering a structured approach to these protocols, the book aims to foster growth in this innovative area of research.