Aptamers for analytical applications

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An essential guide focusing on method developments and applications in aptamers, this resource explores the growing field of aptamer-based systems for various analytical and medical uses. It introduces aptamers, outlines common synthesis protocols, and details optimization strategies for achieving higher affinities to target molecules. Contributions from noted experts provide an in-depth review of aptamer-target interactions and immobilization techniques, discussing method advancements across relevant applications. The book presents various schemes for efficiently immobilizing aptamers on substrates and summarizes characterization methods for aptamer-ligand complexes. It covers aptamer-based analytical methods, including colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow, and non-labeling techniques. Additionally, it reflects on state-of-the-art and emerging applications of aptamer-based methods. This important resource offers a guide to highly specific and sensitive molecular recognition, with affinities comparable to antibodies but at a lower production cost. It discusses analytical method developments and improvements while providing a comprehensive overview of relevant application areas. Written for analytical chemists, biochemists, and researchers, this book adopts a methodological perspective on aptamer generation and modification, emphasizing method developments for practical applications.