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This thesis investigates the impact of propionate (C3) and β-hydroxybutyrate (BHB) on mRNA levels of reference and energy balance-related genes in bovine adipose tissue explants. It explores their differential effects, identifies stable reference genes for qPCR normalization, and establishes a primary preadipocyte culture to assess gene expression during differentiation.
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Expression of genes related to energy balance in adipose tissue of dairy cattle: effects of SCFA on mRNA abundance as quantified by qPCR and relevance of appropriate selection, Afshin Hosseini
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- 2010
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